Fig. 3. GA prevents HDFs from UV-B induced Cyclobutane Pyrimidine dimmers (CPD) & apoptosis. UV-B irradiation to HDFs induces significant CPD's whereas GA pre-treatment protects the HDFs from UV-B -irradiation induced DNA adducts in an intensity dependent manner in ELISA quantification, (Fig. 3A), (*#p<0.001 as compared to UV-B treated). GA pre-treatment significantly protects HDFs from UV-B -irradiation induced apoptotic nuclei suggesting its role in protecting HDFs from UV-B induced DNA fragmentation, (Fig. 3B). GA decreases the TUNNEL positive cells in UV-B -irradiated HDFs in an intensity dependent manner by double fold at 50 µM concentration as compared to UV-B alone, (Fig. 3C), (*#p<0.001 as compared to UV-B treated, **p<0.01 as compared to GA treated).